An analytical method for the quantitative determination of iron ion chelating capacity: development and validation

Iron is a fundamental microelement for human life; however, deficiencies or excesses of these metal ions can cause severe complications and mortality. Chelators are compounds that bind inhibit iron. Ultraviolet-visible (UV-vis) spectrophotometric methods key analytical tools in the identification chemical entities, with benefits having good precision accuracy, equipment being easily available as well quick simple to implement. In this study, we aimed provide an alternative, cheaper method quantification iron ion chelation by substituting ferrozine gallic acid validating its use UV-vis according official ANVISA ICH guidelines. The parameters assessed were specificity, linearity, precision, robustness, finally, percentage chelating was calculated. results demonstrated accurate, simple, specific, selective, precise, reproducible, successfully validated determination chelating. chelating, promoted standard chelator EDTA, 45% 47% Fe2+ Fe3+, respectively. It concluded new beneficial terms simplicity, rapidness, low cost, fact it produces very levels dangerous residues.